Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism
| Autor: | Lia Paggi, S.C. Webb, Stefano D'Amelio, M Picanço, Kostas D. Mathiopoulos, Cláudia Portes Santos, O.N Pugachev |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Genetic Markers
Genetics biology TaqI Anisakis simplex biology.organism_classification DNA Ribosomal Polymerase Chain Reaction Anisakis chemistry.chemical_compound Restriction enzyme Endonuclease Infectious Diseases chemistry Genetic marker Ascaridoidea biology.protein Animals Parasitology Restriction fragment length polymorphism Ribosomal DNA Polymorphism Restriction Fragment Length |
| Zdroj: | International Journal for Parasitology. 30:223-226 |
| ISSN: | 0020-7519 |
| DOI: | 10.1016/s0020-7519(99)00178-2 |
| Popis: | Polymerase-chain-reaction-based restriction fragment length polymorphism analysis was performed to establish genetic markers in rDNA, for the identification of the three sibling species of the Anisakis simplex complex and morphologically differentiated Anisakis species, i.e. Anisakis physeteris, Anisakis schupakovi, Anisakis typica and Anisakis ziphidarum. Different restriction patterns were found between A. simplex sensu stricto and Anisakis pegreffii with two of the restriction endonucleases used (HinfI and TaqI), between A. simplex sensu stricto and A. simplex C with one endonuclease (HhaI), and between A. simplex C and Aniskis pegreffii with three endonucleases (HhaI, HinfI and TaqI), while no variation in patterns was detected among individuals within each species. The species A. physeteris, A. schupakovi, A. typica and A. ziphidarum were found to be different from each other and different from the three sibling species of the A. simplex complex by distinct fragments using 10-12 of the endonucleases tested. The polymorphisms obtained by restriction fragment length polymorphisms have provided a new set of genetic markers for the accurate identification of sibling species and morphospecies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect