Bone Targeted Delivery of SDF-1 via Alendronate Functionalized Nanoparticles in Guiding Stem Cell Migration
| Autor: | Haobo Pan, Qingchang Chen, Shaoquan Bian, Xiaoli Zhao, Yanqun Li, Chuping Zheng, William W. Lu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Stromal cell Materials science Gene delivery Bone and Bones 03 medical and health sciences Mice 0302 clinical medicine Cell Movement medicine Animals General Materials Science Bone regeneration Alendronate Stem Cells Mesenchymal stem cell Mesenchymal Stem Cells Chemokine CXCL12 Transplantation 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research Nanoparticles Bone marrow Stem cell Homing (hematopoietic) |
| Zdroj: | ACS applied materialsinterfaces. 10(28) |
| ISSN: | 1944-8252 |
| Popis: | Stem cells are well-known for their great capacity for tissue regeneration. This provides a promising source for cell-based therapies in treating various bone degenerative disorders. However, the major hurdles for their application in transplantation are the poor bone marrow homing and engraftment efficiencies. Stromal cell-derived factor 1 (SDF-1) has been identified as a major stem cell homing factor. With the aims of bone targeted SDF-1 delivery and regulating MSCs migration, alendronate modified liposomal nanoparticles (Aln-Lipo) carrying SDF-1 gene were developed in this study. Alendronate modification significantly increased the mineral binding affinity of liposomes, and facilitated the gene delivery to osteoblastic cells. Up-regulated SDF-1 expression in osteoblasts triggered MSCs migration. Systemic infusion of Aln-Lipo-SDF-1 with fluorescence labeling in mice showed the accumulation in osseous tissue by biophotonic imaging. Corresponding to the delivered SDF-1, the transplanted GFP+ MSCs were attracted to bone marrow and contributed to bone regeneration. This study may provide a useful technique in regulating stem cell migration. |
| Databáze: | OpenAIRE |
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