Characterization and physiologic regulation of atrial natriuretic factor receptors in rabbit preglomerular renal microvessels
| Autor: | Michael A. Kirschenbaum, Shinya Nakamura, Anshumali Chaudhari |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Afferent arterioles Normal diet Kidney Glomerulus Radioimmunoassay Receptors Cell Surface In Vitro Techniques Binding Competitive General Biochemistry Genetics and Molecular Biology Microcirculation Radioligand Assay Atrial natriuretic peptide Internal medicine otorhinolaryngologic diseases medicine Animals General Pharmacology Toxicology and Pharmaceutics Receptor Desoxycorticosterone Cyclic GMP Kidney Lagomorpha biology urogenital system Chemistry General Medicine biology.organism_classification Endocrinology medicine.anatomical_structure Guanylate Cyclase cardiovascular system Rabbits Receptors Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists Atrial Natriuretic Factor Interlobular arteries |
| Zdroj: | Life sciences. 48(5) |
| ISSN: | 0024-3205 |
| Popis: | There has been no direct demonstration of the presence of guanylate cyclase-linked atrial natriuretic factor receptors in renal preglomerular microvasculature. Using [125I]ANF, we have demonstrated the presence of high affinity (Kd = 80 pM) and low affinity (Kd = 7.2 nM) ANF receptors in membranes derived from rabbit renal preglomerular microvessels (afferent arterioles and interlobular arteries). These microvessels also exhibited the presence of particulate bound ANF-sensitive guanylate cyclase. The density of the high affinity ANF receptor in desoxycorticosterone-treated rabbits on a high-salt diet (31 +/- 3 fmol/mg protein) was nearly half of that seen in rabbits on a normal diet (53 +/- 4 fmol/mg protein; p less than 0.01, n = 4). Data from this study demonstrated the presence of renal preglomerular ANF receptors and suggested that these receptors (perhaps in addition to glomerular ANF receptors) may participate in the regulation of extracellular volume. |
| Databáze: | OpenAIRE |
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