Decreased measles virus-neutralizing activity in sera from otosclerotic patients
| Autor: | Stanimir Kyurkchiev, George Edrev, Petya Zarzalanova, Stephan R. Lolov |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Paramyxoviridae Tetrazolium Salts Antibodies Viral Measles Neutralization Virus Cell Line Measles virus Morbillivirus Neutralization Tests Medicine Humans Mononegavirales Analysis of Variance biology business.industry medicine.disease biology.organism_classification Virology Otosclerosis Otorhinolaryngology Case-Control Studies Immunoglobulin G Immunology biology.protein Female Indicators and Reagents Antibody business |
| Zdroj: | ORL; journal for oto-rhino-laryngology and its related specialties. 65(5) |
| ISSN: | 0301-1569 |
| Popis: | Hypothesis: Any kind of depressed systemic anti-measles reaction can lead to the induction of a local immune response in the inner ear and possibly to reactivation of bone turnover in this region. Methods: Different dilutions of sera were tested for neutralizing activity against a constant viral concentration. The ability of measles virus to infect and replicate in the cell monolayer was detected by enumeration of living and growing cells with a colored reaction. Results: Virus-neutralizing activity in the sera of patients with confirmed otosclerosis was significantly weaker than in that of healthy controls. When age- and sex-matched pairs were compared, the neutralizing activity in the healthy counterpart was higher in 5 cases. Nearly complete viral neutralization was achieved with samples containing inactivated complement and in IgG-containing fractions, but not in immunoglobulin-depleted samples. Conclusion: The present study is consistent with measles virus participation at least in the initiation of some cases of otosclerosis. |
| Databáze: | OpenAIRE |
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