Dopamine-loaded liposome and its application in electrochemical DNA biosensor
| Autor: | Esmaeel Alipour, S. M. Golabi, Hamed Hamishehkar, Tohid Mahmoudi-Badiki |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Sucrose
Dopamine Oligonucleotides Biomedical Engineering Biotin Biosensing Techniques 02 engineering and technology Electrochemistry 01 natural sciences Biomaterials chemistry.chemical_compound medicine Particle Size Electrodes Liposome Chromatography Nanotubes Carbon Phosphatidylethanolamines 010401 analytical chemistry DNA Electrochemical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Freeze Drying chemistry Liposomes 0210 nano-technology Biosensor medicine.drug |
| Zdroj: | Journal of Biomaterials Applications. 31:273-282 |
| ISSN: | 1530-8022 0885-3282 |
| DOI: | 10.1177/0885328216650378 |
| Popis: | In this study, disruption and lyophilization–rehydration of dopamine-loaded liposome and its application in electrochemical DNA biosensor was investigated. The liposomes containing soyphosphatidylcholine and cholesterol were prepared through thin-layer hydration. First, an investigation was carried out to find an appropriate lysing agent for disruption of prepared liposomes. Differential pulse voltammetry, as a high sensitive electrochemical technique, was used along with a multi-walled carbon nanotubes modified glassy carbon electrode for sensitive electrochemical detection of released dopamine from disrupted liposomes. Various lysing agents were investigated and finally, the disruption of liposomes using methanol was selected without any surfactant, because of its least fouling effect. Then, lyophilization of dopamine-loaded liposomes was carried out using sucrose as cryoprotectant. The electrochemical studies of lyophilized liposomes showed that the remained dopamine in sucrose-protected liposomes was higher than sucrose-free liposomes. Furthermore, sucrose has no interference in electrochemical studies. Then, with the addition of biotin-X-DHPE to liposome formulation, the lyophilized sucrose protected dopamine-loaded biotin-tagged liposomes were prepared and the feasibility of application of them in electrochemical DNA biosensor was investigated as signal enhancer and verified for detection of oligonucleotides. |
| Databáze: | OpenAIRE |
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