Pectobacterium atrosepticum Biosensor for Monitoring Blackleg and Soft Rot Disease of Potato
| Autor: | Masoud Bahar, Elisabetta Primiceri, Maria Serena Chiriacò, Giuseppe Maruccio, Palmiro Poltronieri, Mahdis Hashemi Tameh |
|---|---|
| Přispěvatelé: | Tameh, M. H., Primiceri, E., Chiriaco, M. S., Poltronieri, P., Bahar, M., Maruccio, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Biotechnology
Clinical Biochemistry Blackleg Early detection 02 engineering and technology Biology 01 natural sciences Article phytopathogen lcsh:TP248.13-248.65 Lab-on-chip Pectobacterium atrosepticum Phytopathogen business.industry 010401 analytical chemistry food and beverages General Medicine Standard methods 021001 nanoscience & nanotechnology biosensors Soft rot disease 0104 chemical sciences Biotechnology lab-on-chip 0210 nano-technology business Biosensor |
| Zdroj: | Biosensors Volume 10 Issue 6 Biosensors, Vol 10, Iss 64, p 64 (2020) Biosensors (Basel) 10 (2020): 1–12. doi:10.3390/bios10060064 info:cnr-pdr/source/autori:Mahdis Hashemi Tameh, Elisabetta Primiceri, Maria Serena Chiriacò, Palmiro Poltronieri, Masoud Bahar, Giuseppe Maruccio/titolo:Pectobacterium atrosepticum Biosensor for Monitoring Blackleg and Soft Rot Disease of Potato/doi:10.3390%2Fbios10060064/rivista:Biosensors (Basel)/anno:2020/pagina_da:1/pagina_a:12/intervallo_pagine:1–12/volume:10 |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios10060064 |
| Popis: | Pectobacterium atrosepticum (Pba) is a quarantine and threatening phytopathogen known as the causal agent of blackleg and soft rot disease of potatoes in many areas. Its early detection is then important to have healthy potato tubers and reduce economic losses. Today, conventional methods such as enzyme-linked immunosorbent-assay (ELISA) and polymerase chain reaction (PCR) are typically used for Pba detection, but they are expensive and time-consuming. Here we report on the optimization of an alternative approach based on an electrochemical impedance immunosensor combining a microfluidic module and a microelectrodes array, and having advantages in terms of low cost, ease of use and portability. For validation and for assessing its performance, the lab-on-chip platform has been compared with two standard methods (ELISA and PCR). |
| Databáze: | OpenAIRE |
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