Whole-Animal Senescent Cytotoxic T Cell Removal Using Antibodies Linked to Magnetic Nanoparticles
| Autor: | Timothy Webb, Justin Rebo, John Schloendorn, Ben Zealley, Keith Causey, Mark Hamalainen, Brian Cook |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Aging
Lymphokine-activated killer cell Cell Separation CD8-Positive T-Lymphocytes Biology Natural killer T cell Cell biology Cytapheresis Mice Inbred C57BL Magnetics Mice Interleukin 21 Immune system Immunology Animals Nanoparticles Cytotoxic T cell Lectins C-Type IL-2 receptor Receptors Immunologic Geriatrics and Gerontology Antigen-presenting cell CD8 Antilymphocyte Serum T-Lymphocytes Cytotoxic |
| Zdroj: | Rejuvenation Research. 13:298-300 |
| ISSN: | 1557-8577 1549-1684 |
| DOI: | 10.1089/rej.2009.0964 |
| Popis: | A major type of unwanted cells that accumulate in aging are anergic cytotoxic T cells. These cells often have virus-specific T cell receptors, as well as other surface markers that distinguish them from their youthful counterparts, and they are thought to play a major role in the decline of the immune system with age. Here we consider two surface markers thought to define these cells in mice, CD8 and Killer cell lectin-like receptor G1 (KLRG1), and a means we developed to remove these cells from the blood of aged C57BL/6 mice. Using antibodies with magnetic nanoparticles linked to their Fc domains, we first developed a method to use magnets to filter out the unwanted cells from the blood and later constructed a device that does this automatically. We demonstrated that this device could reduce the KLRG1-positive CD8 cell count in aged mouse blood by a factor of 7.3 relative to the total CD8 cell compartment, reaching a level typically seen only in very young animals. |
| Databáze: | OpenAIRE |
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