Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion
| Autor: | Lentini, Gaëlle, Ben Chaabene, Rouaa, Vadas, Oscar, Ramakrishnan, Chandra, Mukherjee, Budhaditya, Mehta, Ved, Lunghi, Matteo, Grossmann, Jonas, Maco, Bohumil, Visentin, Rémy, Hehl, Adrian B, Korkhov, Volodymyr M, Soldati-Favre, Dominique |
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| Přispěvatelé: | University of Zurich, Korkhov, Volodymyr M, Soldati-Favre, Dominique |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
10078 Institute of Parasitology
Proteomics Virulence Factors Science Protozoan Proteins General Physics and Astronomy 610 Medicine & health 1600 General Chemistry Genetics and Molecular Biology Kinases Article Host-Parasite Interactions 1300 General Biochemistry Genetics and Molecular Biology Cryoelectron microscopy 600 Technology parasitic diseases Humans Cells Cultured ddc:616 Secretory Pathway Cell Membrane Receptor Protein-Tyrosine Kinases Biological Transport General Chemistry 3100 General Physics and Astronomy Parasite biology General Biochemistry 570 Life sciences biology Toxoplasma Toxoplasmosis |
| Zdroj: | Nature Communications, Vol. 12, No 1 (2021) P. 3788 Nature Communications, 12 (1) Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021) |
| ISSN: | 2041-1723 |
| Popis: | Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete their rhoptry content and insert a complex of RON proteins on the cytoplasmic side of the host cell membrane providing an anchor to which the parasite tethers. Here we show that a rhoptry-resident kinase RON13 is a key virulence factor that plays a crucial role in host cell entry. Cryo-EM, kinase assays, phosphoproteomics and cellular analyses reveal that RON13 is a secretory pathway kinase of atypical structure that phosphorylates rhoptry proteins including the components of the RON complex. Ultimately, RON13 kinase activity controls host cell invasion by anchoring the moving junction at the parasite-host cell interface. Nature Communications, 12 (1) ISSN:2041-1723 |
| Databáze: | OpenAIRE |
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