Myostatin Knockout in Mice Increases Myogenesis and Decreases Adipogenesis
| Autor: | Heather B. Arnold, Mary Anne Della-Fera, Michael J. Azain, Ji Lin, Clifton A. Baile, Diane L. Hartzell |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Leptin
Male medicine.medical_specialty Biophysics Receptors Cytoplasmic and Nuclear Adipose tissue Myostatin Muscle Development Biochemistry Eating Mice Transforming Growth Factor beta Enhancer binding Internal medicine CCAAT-Enhancer-Binding Protein-alpha medicine Animals RNA Messenger Molecular Biology Mice Knockout biology Myogenesis Body Weight Wild type Cell Biology Kinetics Endocrinology Adipose Tissue Adipogenesis Knockout mouse Body Composition biology.protein Female Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 291:701-706 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2002.6500 |
| Popis: | Growth differentiation factor-8 (GDF-8), or Myostatin, plays an important inhibitory role during muscle development. Since muscle and adipose tissue develop from the same mesenchymal stem cells, we hypothesized that Myostatin gene knockout may cause a switch between myogenesis and adipogenesis. Male and female wild type (WT) and Myostatin knockout (KO) mice were sacrificed at 4, 8, and 12 weeks of age. The gluteus muscle (GM) was larger in KO mice compared to WT mice at 8 (P < 0.01) and 12 (P < 0.001) weeks. At 12 weeks, KO mice had decreased fat depots (P < 0.01). Compared to 12-week-old WT mice, serum leptin concentration in KO mice was lower (P < 0.001) and leptin mRNA expression was decreased (P < 0.01) in inguinal adipose tissue. CCAAT/enhancer binding protein-alpha (C/EBPalpha) and peroxisome proliferator-activated receptor-gamma (PPARgamma) levels in adipose tissue were significantly lower in KO mice compared to WT mice. Thus, increased muscle development in Myostatin knockout mice is associated with reduced adipogenesis and consequently, decreased leptin secretion. |
| Databáze: | OpenAIRE |
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