Decreased fibronectin production significantly contributes to dysregulated repair of asthmatic epithelium
| Autor: | Teal S. Hallstrand, Christopher Taplin, Richard P. Beyer, Michael S. Kobor, Stephen M. Stick, Peter D. Paré, Paul T. Stevens, Darryl A. Knight, Erika N. Sutanto, Anthony Kicic |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pulmonary and Respiratory Medicine
Adolescent Inflammation Enzyme-Linked Immunosorbent Assay Respiratory Mucosa Critical Care and Intensive Care Medicine Hydroxamic Acids Dexamethasone Extracellular matrix Transforming Growth Factor beta1 Intensive care medicine Hypersensitivity Gene silencing Humans Cycloheximide Child Cells Cultured biology Homocystine Microarray analysis techniques business.industry Epithelial Cells respiratory system DNA Methylation Microarray Analysis Epithelium Asthma respiratory tract diseases Extracellular Matrix Fibronectins A. Asthma and Allergy Fibronectin medicine.anatomical_structure Child Preschool Immunology biology.protein Azacitidine Respiratory epithelium Tetradecanoylphorbol Acetate medicine.symptom business |
| Zdroj: | American journal of respiratory and critical care medicine. 181(9) |
| ISSN: | 1535-4970 |
| Popis: | Damage to airway epithelium is followed by deposition of extracellular matrix (ECM) and migration of adjacent epithelial cells. We have shown that epithelial cells from children with asthma fail to heal a wound in vitro.To determine whether dysregulated ECM production by the epithelium plays a role in aberrant repair in asthma.Airway epithelial cells (AEC) from children with asthma (n = 36), healthy atopic control subjects (n = 23), and healthy nonatopic control subjects (n = 53) were investigated by microarray, gene expression and silencing, transcript regulation analysis, and ability to close mechanical wounds.Time to repair a mechanical wound in vitro by AEC from healthy and atopic children was not significantly different and both were faster than AEC from children with asthma. Microarray analysis revealed differential expression of multiple gene sets associated with repair and remodeling in asthmatic AEC. Fibronectin (FN) was the only ECM component whose expression was significantly lower in asthmatic AEC. Expression differences were verified by quantitative polymerase chain reaction and ELISA, and reduced FN expression persisted in asthmatic cells over passage. Silencing of FN expression in nonasthmatic AEC inhibited wound repair, whereas addition of FN to asthmatic AEC restored reparative capacity. Asthmatic AEC failed to synthesize FN in response to wounding or cytokine/growth factor stimulation. Exposure to 5', 2'deoxyazacytidine had no effect on FN expression and subsequent analysis of the FN promoter did not show evidence of DNA methylation.These data show that the reduced capacity of asthmatic epithelial cells to secrete FN is an important contributor to the dysregulated AEC repair observed in these cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|