Detection and quantification of 3,5,3'-triiodothyronine and 3,3',5'-triiodothyronine by electrospray ionization tandem mass spectrometry
Autor: | Abigail H. Conrad, Yuntao Zhang, Gary W. Conrad |
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Rok vydání: | 2005 |
Předmět: |
Spectrometry
Mass Electrospray Ionization Chromatography Protein mass spectrometry Chemistry Electrospray ionization Microchemistry Selected reaction monitoring Analytical chemistry Extractive electrospray ionization Mass spectrometry Capillary electrophoresis–mass spectrometry Sample preparation in mass spectrometry Isomerism Structural Biology Triiodothyronine Direct electron ionization liquid chromatography–mass spectrometry interface Spectroscopy Algorithms |
Zdroj: | Journal of the American Society for Mass Spectrometry. 16(11) |
ISSN: | 1044-0305 |
Popis: | A novel and rapid method for identifying and quantifying 3,5,3'-triiodothyronine (T3) and 3,3',5'-triiodothyronine (rT3; reverse T3) has been introduced using electrospray ionization tandem mass spectrometry (ESI-MS/MS). MS(2) spectra in either negative ionization mode or positive ionization mode can be used to differentiate T3 and rT3. Quantification of the T3 and rT3 isomers under the negative ionization mode is also achieved without prior separation by HPLC. |
Databáze: | OpenAIRE |
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