Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection

Autor: Ricardo Luís Mayer Weber, Milena Schenkel Homrich, Atilio Pedro Castagnaro, Beatriz Wiebke-Strohm, Mayra Costa da Cruz Gallo de Carvalho, Francismar Corrêa Marcelino-Guimarães, Caroline Cabreira, Estefania Mancini, Gaston Westergaard, Lauro Bücker-Neto, Renata Stolf, Marta Bencke-Malato, Ricardo Vilela Abdelnoor, Márcia Margis-Pinheiro, Maria Helena Bodanese-Zanettini, Andreia Carina Turchetto-Zolet, Marina Borges Osorio
Přispěvatelé: MARTA BENCKE-MALATO, UFRGS, CAROLINE CABREIRA, UFRGS, BEATRIZ WIEBKE-STROHM, UFRGS, LAURO BÜCKER-NETO, UFRGS, ESTEFANIA MANCINI, Instituto de Agrobiotecnologia Rosario SA, MARINA B. OSORIO, UFRGS, MILENA S. HOMRICH, UFRGS, ANDREIA CARINA TURCHETTO-ZOLET, UFRGS, MAYRA C. C. G. DE CARVALHO, CNPSO - estagiária, RENATA STOLF, CNPSO - estagiària, RICARDO L. M. WEBER, UFRGS, GASTÓN WESTERGAARD, Instituto de Agrobiotecnologia Rosario SA, ATÍLIO P. CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres (EEAOC), RICARDO VILELA ABDELNOOR, CNPSO, FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO, MÁRCIA MARGIS-PINHEIRO, UFRGS, MARIA HELENA BODANESE-ZANETTINI, UFRGS.
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Soja
Otras Biotecnología Agropecuaria
Glycine max
Plant Science
Genome
Genetic transformation
Gene Expression Regulation
Plant
FUNCTIONAL ANALYSIS
FUNGUS RESISTANCE
Genotype
Plant Proteins
Genetics
purl.org/becyt/ford/4.4 [https]
food and beverages
Asian Soybean Rust
GLYCINE MAX
Plants
Genetically Modified
TRANSCRIPTION FACTORS
Phakopsora pachyrhizi
Host-Pathogen Interactions
Disease Susceptibility
Genome
Plant
Research Article
ASIAN SOYBEAN RUST
Pseudogene
Biotecnología Agropecuaria
Molecular Sequence Data
Fungus resistance
Biology
Soybeans
Transformation
Genetic
Botany
Consensus Sequence
Transcription factors
Regeneration
Amino Acid Sequence
Gene Silencing
Gene
Plant Diseases
Functional analysis
Basidiomycota
Gene Expression Profiling
fungi
Molecular Sequence Annotation
biology.organism_classification
GENETIC TRANSFORMATION
WRKY protein domain
Gene expression profiling
Plant Leaves
Transformation (genetics)
CIENCIAS AGRÍCOLAS
Sequence Alignment
purl.org/becyt/ford/4 [https]
Zdroj: BMC Plant Biology
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1471-2229
Popis: Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants. Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil
Databáze: OpenAIRE
Externí odkaz: