Characterization of Ineffective Erythropoiesis in Erythroleukaemia
| Autor: | U. Kempgens, W. Queisser, U. Müller, U. Pepperl |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
Ineffective erythropoiesis Erythroblasts Mitosis Biology medicine.disease_cause Polyploidy hemic and lymphatic diseases Labelling medicine Humans Erythropoiesis Feulgen stain Aged DNA synthesis hemic and immune systems DNA Hematology General Medicine Middle Aged Diploidy Molecular biology Cytophotometry Basophils Blood Cell Count Immunology Female Leukemia Erythroblastic Acute circulatory and respiratory physiology |
| Zdroj: | Acta Haematologica. 54:65-74 |
| ISSN: | 1421-9662 0001-5792 |
| DOI: | 10.1159/000208054 |
| Popis: | In 6 cases initially showing a symptomatology of erythroleukemia, the proliferation of erythroblasts was studied by combined Feulgen cytophotometry and autoradiography after labelling with 3H-TdR in vivo. A proliferation disturbance was observed consisting of an accumulation of diploid and unlabelled cells and a decreased proportion of cells in S. This defect occurred within the basophilic cell compartment in one case and within the early polychromatic cell compartment in all 6 cases. The results indicate the existence of out-of-cycle cells, which may be responsible for the inefficacy for erythropoiesis in this disease. The defect was present in all 4 cases with acute erythroleukaemia. In one of the two cases showing no deterioration of the physical and haematological condition over a period of years, an additional defect was observed, consisting of a striking accumulation of tetraploid unlabelled cells. Therefore, the technique used may be suitable as a diagnostic tool for evaluation of new types of ineffective erythropoiesis. |
| Databáze: | OpenAIRE |
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