SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization

Autor: Philippe P. Roux, Elias H. Barriga, Guillaume Romet-Lemonne, Aurélie Bertin, Antoine Jégou, Guillaume Charras, Ewa K. Paluch, Emma Ferber, Art Alberts, Roberto Mayor, Amina Yonis, Miia Bovellan, Geneviève Lavoie, Matthew B. Smith, Antoine Méant, Malti Vaghela, Luyan Cao, Priyamvada Chugh, Julien Maufront
Přispěvatelé: Institut Jacques Monod (IJM (UMR_7592)), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), University College of London [London] (UCL), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Bertin, Aurélie [0000-0002-3400-6887], Mayor, Roberto [0000-0001-9053-9613], Roux, Philippe P [0000-0002-5962-0250], Jégou, Antoine [0000-0003-0356-3127], Romet-Lemonne, Guillaume [0000-0002-4938-1065], Charras, Guillaume [0000-0002-7902-0279], Apollo - University of Cambridge Repository
Rok vydání: 2019
Předmět:
Zdroj: Nature Cell Biology
Nature Cell Biology, Nature Publishing Group, 2020, 22, pp.803-814. ⟨10.1038/s41556-020-0531-y⟩
ISSN: 1476-4679
1465-7392
DOI: 10.1038/s41556-020-0531-y⟩
Popis: International audience; Cell shape is controlled by the submembranous cortex, an actomyosin network mainly generated by two actin nucleators: the Arp2/3 complex and the formin mDia1. Changes in relative nucleator activity may alter cortical organization, mechanics and cell shape. Here we investigate how nucleation-promoting factors mediate interactions between nucleators. In vitro, the nucleation-promoting factor SPIN90 promotes formation of unbranched filaments by Arp2/3, a process thought to provide the initial filament for generation of dendritic networks. Paradoxically, in cells, SPIN90 appears to favour a formin-dominated cortex. Our in vitro experiments reveal that this feature stems mainly from two mechanisms: efficient recruitment of mDia1 to SPIN90–Arp2/3 nucleated filaments and formation of a ternary SPIN90–Arp2/3–mDia1 complex that greatly enhances filament nucleation. Both mechanisms yield rapidly elongating filaments with mDia1 at their barbed ends and SPIN90–Arp2/3 at their pointed ends. Thus, in networks, SPIN90 lowers branching densities and increases the proportion of long filaments elongated by mDia1.
Databáze: OpenAIRE
Externí odkaz: