Low doses of IgG from atopic individuals can modulate in vitro IFN-γ production by human intra-thymic TCD4 and TCD8 cells: An IVIg comparative approach
| Autor: | Aline Aparecida de Lima Lira, Luciana Bento-de-Souza, Fábio da Ressureição Sgnotto, Jefferson Russo Victor, Marília Garcia de Oliveira, Alberto José da Silva Duarte |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Hypersensitivity Immediate Allergy medicine.medical_treatment Immunology Cell Thymus Gland CD8-Positive T-Lymphocytes Peripheral blood mononuclear cell 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immunology and Allergy Medicine Humans Immunologic Factors Interferon gamma Pharmacology Thymocytes biology business.industry Infant Newborn Immunoglobulins Intravenous medicine.disease Research Papers In vitro Coculture Techniques body regions 030104 developmental biology Cytokine medicine.anatomical_structure biology.protein Antibody business CD8 030215 immunology medicine.drug |
| Zdroj: | Human vaccinesimmunotherapeutics. 13(7) |
| ISSN: | 2164-554X |
| Popis: | The regulatory effect of allergic responses induced by IgG antibodies on human intra-thymic cells has not been reported in the literature. The aim of this study was to evaluate the possible differential effect of purified IgG from atopic and non-atopic individuals on human intra-thymic αβT cell cytokine production. Thymic tissues were obtained from 14 patients who were less than 7 d old. Additionally, blood samples were collected from atopic and non-atopic volunteers. Thymocytes and peripheral blood mononuclear cells were cultured with purified atopic or non-atopic IgG, and intracellular cytokine production was assessed. Purified IgG did not influence the frequency or viability of human intra-thymic αβT cells. Purified non-atopic IgG induced greater IFN-γ production by intra-thymic CD4+CD8+ T cells than did the mock treatment and atopic IgG. A similar effect of purified non-atopic IgG on TCD8 cells was observed compared with the mock treatment. Atopic IgG inhibited IFN-γ and TGF-β production by intra-thymic TCD4 cells. Treatment with intravenous immunoglobulin resulted in intermediate levels of IFN-γ and TGF-β in intra-thymic TCD4 cells compared with treatment with atopic and non-atopic IgG. Peripheral TCD4 cells from non-atopic individuals produced IFN-γ only in response to atopic IgG. This report describes novel evidence revealing that IgG from atopic individuals may influence intracellular IFN-γ production by intra-thymic αβT cells in a manner that may favor allergy development. |
| Databáze: | OpenAIRE |
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