Generation of mice with a conditional allele for the p75NTR neurotrophin receptor gene
| Autor: | Jieun Kim, Qianfeng Li, Penny S. Thomas, Vesa Kaartinen, Brian A. Pierchala, Liang tung Yang, Emil Bogenmann |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
musculoskeletal diseases Genotype Genetic Vectors Mutant Embryonic Development Cre recombinase Receptors Nerve Growth Factor Biology Article Mice Exon Endocrinology Conditional gene knockout Genetics Animals Cloning Molecular Allele skin and connective tissue diseases Gene Alleles Crosses Genetic Mice Knockout Reflex Abnormal Peripheral Nervous System Diseases Exons Cell Biology Embryo Mammalian Immunohistochemistry biological factors Lower Extremity nervous system Neural Crest Knockout mouse biology.protein Female sense organs Neurotrophin |
| Zdroj: | genesis. 49:862-869 |
| ISSN: | 1526-954X |
| DOI: | 10.1002/dvg.20747 |
| Popis: | The p75(NTR) neurotrophin receptor has been implicated in multiple biological and pathological processes. While significant advances have recently been made in understanding the physiologic role of p75(NTR) , many details and aspects remain to be determined. This is in part because the two existing knockout mouse models (Exons 3 or 4 deleted, respectively), both display features that defy definitive conclusions. Here we describe the generation of mice that carry a conditional p75(NTR) (p75(NTR-FX) ) allele made by flanking Exons 4-6, which encode the transmembrane and all cytoplasmic domains, by loxP sites. To validate this novel conditional allele, both neural crest-specific p75(NTR) /Wnt1-Cre mutants and conventional p75(NTR) null mutants were generated. Both mutants displayed abnormal hind limb reflexes, implying that loss of p75(NTR) in neural crest-derived cells causes a peripheral neuropathy similar to that seen in conventional p75(NTR) mutants. This novel conditional p75(NTR) allele will offer new opportunities to investigate the role of p75(NTR) in specific tissues and cells. |
| Databáze: | OpenAIRE |
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