Gold nanoparticles loaded TNF-α and CALNN peptide as a drug delivery system and promising therapeutic agent for breast cancer cells
| Autor: | Majid S. Jabir, Noor A. Abood, Marwa H. Jawad, Kamile Öztürk, Haider Kadhim, Salim Albukhaty, Ahmed Al-Shammari, Faizah A. AlMalki, Jawaher Albaqami, Ghassan M. Sulaiman |
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| Přispěvatelé: | Sabire Yazıcı Fen Edebiyat Fakültesi |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Materials Technology. 37:3152-3166 |
| ISSN: | 1753-5557 1066-7857 |
| Popis: | We investigated the anti-cancer properties of gold nanoparticles loaded TNF- and CALNN peptides, which we proposed as a potential drug delivery system using in vitro and in vivo models. The binding of GNPs-TNF- and GNPs-TNF-CALNN was characterized using a UV, ELISA and SEM analysis. The outcomes demonstrated that a novel drug delivery system had an anti-proliferative activity against breast cancer cell lines through a mechanism of apoptosis induction. In vivo model involved studying the cytotoxic influence of a drug delivery system GNPs, GNPs-TNF-alpha and GNPs-TNF-alpha-CALNN when applied to the transplanted AN-3 cell line. tumor sections were examined using microarray. In-vivo studies demonstrated that GNPs alone had less of a growth inhibitory effect on tumors implanted in mice when compared to GNPs-TNF-CALNN combined therapy. The cytotoxic assay showed that GNPs, GNPs-TNF-alpha and GNPs-TNF-alpha-CALNN exhibit selective toxicity towards cancer cells, inducing cell apoptosis through activation of caspase-3 and 7, p53 protein. |
| Databáze: | OpenAIRE |
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