Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML

Autor:	Kazunori Nakase, Naoyuki Katayama, Rino Arakawa, Kohshi Ohishi, Masahiro Masuya, Isao Tawara, Hiroshi Miwa, Yuki Kageyama
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Male Myeloid Physiology CD34 Gene Expression Antigens CD34 CD38 Hematologic Cancers and Related Disorders Spectrum Analysis Techniques hemic and lymphatic diseases Immune Physiology Medicine and Health Sciences IL-2 receptor Cells Cultured education.field_of_study Innate Immune System Multidisciplinary Myeloid leukemia hemic and immune systems Hematology Middle Aged Myeloid Leukemia Flow Cytometry Gene Expression Regulation Neoplastic Leukemia Phenotypes Leukemia Myeloid Acute medicine.anatomical_structure Oncology Spectrophotometry Neoplastic Stem Cells Medicine Cytokines Female Biological Cultures Cytophotometry psychological phenomena and processes Research Article Acute Myeloid Leukemia Adult Science Population Immunology chemical and pharmacologic phenomena Ligation Independent Cloning Mice Transgenic Biology Research and Analysis Methods 03 medical and health sciences Young Adult mental disorders Leukemias medicine Genetics Animals Humans education Molecular Biology Techniques Molecular Biology Aged Interleukin-2 Receptor alpha Subunit Cancers and Neoplasms Biology and Life Sciences Cell Cultures Molecular Development medicine.disease Transplantation 030104 developmental biology Immune System Cancer research Neoplasm Transplantation Cloning Molecular Cloning Developmental Biology
Zdroj:	PLoS ONE PLoS ONE, Vol 13, Iss 12, p e0209295 (2018)
ISSN:	1932-6203
Popis:	CD25 is expressed on leukemic cells in 10–20% cases of acute myeloid leukemia (AML), and its expression is associated with poor prognosis. We reevaluated the relationship between CD25 expression and the leukemia-initiating cell (LIC) properties of AML using a patient-derived xenograft model. We divided lineage marker-negative (Lin–) CD34+CD38– or Lin–CD34+ cells from CD25-positive AML into CD25-positive and -negative populations, and then transplanted each population into NOD.Cg-PrkdcscidIl2rgtm1Wjl/Sz mice. Leukemic engraftment was observed with both CD25-positive and -negative populations from three of nine CD25-positive AML patients. In two of those three patients, CD25-positive and -negative Lin–CD34+ cells engrafted at the primary transplantation led to leukemic engraftment at the secondary transplantation, in which engrafted cells contained both CD25-positive and -negative Lin–CD34+ AML cells. In an in vitro culture system, expression of CD25 was considerably induced in the CD25-negative population of Lin–CD34+ cells from two cases of CD25-positive AML. In one case, CD25-positive Lin–CD34+ cells gave rise to CD25-negative as well as -positive CD34+ cells. These observations suggest that there exist CD25-positive and -negative populations that can reconstitute CD25-positive AML in a patient-derived xenograft model, and that CD25 expression fluctuates in the LICs of AML.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85c5f7ba91f538aeb01915195a2b7b5d http://europepmc.org/articles/PMC6294374 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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