CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung
| Autor: | Andreas Hoek, Aichurek Soultanova, Wolfgang Kummer, Krupali V. Poharkar, Sudhanshu Bhushan, Uwe Pfeil, Alexander Perniss, Ulrich Gärtner, Wafaa Mahmoud, Torsten Hain |
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| Rok vydání: | 2021 |
| Předmět: |
Chemokine
Cell type Histology Calcitonin Gene-Related Peptide Cholinergic Agents Pathology and Forensic Medicine Mice Immune system Neuroendocrine Cells medicine Animals RNA Messenger CXCL13 Lung B cell ddc:610 Tracheal Epithelium Innate immune system biology Epithelial Cells Cell Biology Chemokine CXCL13 Cell biology Trachea medicine.anatomical_structure biology.protein Respiratory epithelium |
| Zdroj: | Cell and Tissue Research. 390:35-49 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-021-03552-2 |
| Popis: | The conducting airways are lined by distinct cell types, comprising basal, secretory, ciliated, and rare cells, including ionocytes, solitary cholinergic chemosensory cells, and solitary and clustered (neuroepithelial bodies) neuroendocrine cells. Airway neuroendocrine cells are in clinical focus since they can give rise to small cell lung cancer. They have been implicated in diverse functions including mechanosensation, chemosensation, and regeneration, and were recently identified as regulators of type 2 immune responses via the release of the neuropeptide calcitonin gene-related peptide (CGRP). We here assessed the expression of the chemokine CXCL13 (B cell attracting chemokine) by these cells by RT-PCR, in silico analysis of publicly available sequencing data sets, immunohistochemistry, and immuno-electron microscopy. We identify a phenotype of neuroendocrine cells in the naïve mouse, producing the chemokine CXCL13 predominantly in solitary neuroendocrine cells of the tracheal epithelium (approx. 70% CXCL13+) and, to a lesser extent, in the solitary neuroendocrine cells and neuroepithelial bodies of the intrapulmonary bronchial epithelium (+). In silico analysis of published sequencing data of murine tracheal epithelial cells was consistent with the results obtained by immunohistochemistry as it revealed that neuroendocrine cells are the major source of Cxcl13-mRNA, which was expressed by 68–79% of neuroendocrine cells. An unbiased scRNA-seq data analysis of overall gene expression did not yield subclusters of neuroendocrine cells. Our observation demonstrates phenotypic heterogeneity of airway neuroendocrine cells and points towards a putative immunoregulatory role of these cells in bronchial-associated lymphoid tissue formation and B cell homeostasis. |
| Databáze: | OpenAIRE |
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