Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis
| Autor: | Friedhelm Meinhardt, Susanne Wieland, Renée Eichstädt, Jens Waldeck, Heike Meyer-Rammes, Hannes Nahrstedt |
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| Rok vydání: | 2007 |
| Předmět: |
Genetics
Bacillaceae Models Genetic Ultraviolet Rays Operon Mutant Wild type Bacillus General Medicine Biology biology.organism_classification Polymerase Chain Reaction Applied Microbiology and Biotechnology Blotting Southern Industrial Microbiology Genes Bacterial Mutation Bacillus licheniformis SOS response SOS Response Genetics Homologous recombination Gene Deletion Biotechnology Nucleotide excision repair |
| Zdroj: | Applied Microbiology and Biotechnology. 73:1340-1347 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-006-0602-4 |
| Popis: | From a Bacillus licheniformis wild type as well as a defined asporogenous derivative, stable UV hypersensitive mutants were generated by targeted deletion of the uvrBA operon, encoding highly conserved key components of the nucleotide excision repair. Comparative studies, which included the respective parental strains, revealed no negative side effects of the deletion, neither on enzyme secretion nor on vegetative propagation. Thus, the uvrBA locus proved to be a useful deletion target for achieving biological containment in this industrially exploited bacterium. In contrast to recA mutants, which also display UV hypersensitivity, further strain development via homologous recombination techniques will be still possible in such uvr mutants. |
| Databáze: | OpenAIRE |
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