Single-molecule detection on a portable 3D-printed microscope
| Autor: | James W. P. Brown, Dominic J. B. Hunter, Yann Gambin, Emma Sierecki, Akshay Bhumkar, Bauer Arnaud Luc, Katharina Gaus, Mark E. Polinkovsky |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
3d printed
Microscope Prions Computer science Plug and play Science Confocal General Physics and Astronomy Fluorescence correlation spectroscopy Nanotechnology 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Education law.invention 03 medical and health sciences Single-molecule biophysics law Sensitivity (control systems) lcsh:Science 030304 developmental biology 0303 health sciences Multidisciplinary 010405 organic chemistry General Chemistry Amyloid fibril Photon counting 0104 chemical sciences lipids (amino acids peptides and proteins) lcsh:Q Protein aggregation |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-7 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Single-molecule assays have, by definition, the ultimate sensitivity and represent the next frontier in biological analysis and diagnostics. However, many of these powerful technologies require dedicated laboratories and trained personnel and have therefore remained research tools for specialists. Here, we present a single-molecule confocal system built from a 3D-printed scaffold, resulting in a compact, plug and play device called the AttoBright. This device performs single photon counting and fluorescence correlation spectroscopy (FCS) in a simple format and is widely applicable to the detection of single fluorophores, proteins, liposomes or bacteria. The power of single-molecule detection is demonstrated by detecting single α-synuclein amyloid fibrils, that are currently evaluated as biomarkers for Parkinson’s disease, with an improved sensitivity of >100,000-fold over bulk measurements. Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-synuclein aggregation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|