Fatty acid incorporation into membranes of dispersed rat submandibular salivary gland cells and their effect on adenylate cyclase activity
Autor: | S.N. Ahmad, Syed Q. Alam, Bassima S. Alam |
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Rok vydání: | 1990 |
Předmět: |
Male
Membrane lipids Adenylate kinase Oleic Acids Biology Gas Chromatography-Mass Spectrometry Salivary Glands chemistry.chemical_compound Membrane Lipids Membrane fluidity Animals Carbon Radioisotopes General Dentistry Cells Cultured chemistry.chemical_classification Glucosamine Membrane Glycoproteins Cell Membrane Fatty acid Sodium Dodecyl Sulfate Rats Inbred Strains Cell Biology General Medicine Elaidic acid Rats Membrane Otorhinolaryngology Biochemistry chemistry Specific activity Electrophoresis Polyacrylamide Gel Cyclase activity Stearic Acids Adenylyl Cyclases Oleic Acid |
Zdroj: | Archives of oral biology. 35(11) |
ISSN: | 0003-9969 |
Popis: | Dispersed cells from rat submandibular salivary glands were incubated in medium supplemented with different fatty acids, such as stearic (18:0), oleic (cis 18:1 ω9) and elaidic acid (trans 18:1 ω9). The exogenous fatty acids were incorporated into the membrane lipids. A comparison of the adenylate cyclase activity in membranes of cells incubated with different fatty acids revealed the following order (from highest to lowest specific activity): stearic, elaidic, oleic. The findings suggest that changes in adenylate cyclase activity in these dispersed membranes enriched with different fatty acids may be related to the possible changes in membrane fluidity. The synthesis of glycoproteins was studied by measuring the incorporation of [1-14C]-glucosamine into TCA-PTA-precipitable material in the presence or absence of fatty acids. No effect of the exogenous fatty acids on glycoprotein synthesis was observed. |
Databáze: | OpenAIRE |
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