Laserspray ionization using an atmospheric solids analysis probe for sample introduction
| Autor: | Sarah Trimpin, Charles N. McEwen, Frank Zydel |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Atmospheric pressure
Ion-mobility spectrometry Chemistry 010401 analytical chemistry Analytical chemistry 010402 general chemistry Tandem mass spectrometry Mass spectrometry 01 natural sciences Sample preparation in mass spectrometry 0104 chemical sciences Matrix (chemical analysis) Structural Biology Ionization Mass spectrum Spectroscopy |
| Zdroj: | Journal of the American Society for Mass Spectrometry. 21:1889-1892 |
| ISSN: | 1044-0305 |
| DOI: | 10.1016/j.jasms.2010.07.005 |
| Popis: | A newly introduced high sensitivity laserspray (LSI) mass spectrometry (MS) method that uses laser ablation of a matrix/analyte mixture at atmospheric pressure (AP) to obtain multiply charged ions from nonvolatile as well as high-mass compounds is now implemented using a simple probe device. The probe used in the LSI approach was originally designed for sample introduction into an AP ionization source using the atmospheric solids analysis probe (ASAP) method. Multiply charged mass spectra of peptides and proteins in 2,5-dihydroxybenzoic acid matrix were readily obtained on two mass spectrometers from different manufacturers with sample introduction using melting point tubes. Here we demonstrate rapid analysis by placing four peptide and protein samples on a single melting point tube. Mass spectra were obtained at high-resolution and using ion mobility spectrometry/MS. |
| Databáze: | OpenAIRE |
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