Microtubule-dependent ribosome localization in C. elegans neurons

Autor: Yishi Jin, Mark H. Ellisman, Kentaro Noma, Alexandr Goncharov
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
QH301-705.5
1.1 Normal biological development and functioning
Science
Green Fluorescent Proteins
Microtubules
Ribosome
General Biochemistry
Genetics and Molecular Biology
Green fluorescent protein
neuroscience
03 medical and health sciences
Underpinning research
Microtubule
UNC-16/JIP3
Protein biosynthesis
Animals
Biology (General)
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Neurons
Staining and Labeling
General Immunology and Microbiology
biology
Chemistry
General Neuroscience
Signal Transducing
Neurosciences
Adaptor Proteins
Biological Transport
General Medicine
biology.organism_classification
Subcellular localization
Cell biology
030104 developmental biology
nervous system
axon injury
MEC-15/F-box protein
C. elegans
Medicine
Biochemistry and Cell Biology
Ribosome localization
synapse development
Ribosomes
Genetic screen
Zdroj: eLife, Vol 6 (2017)
Popis: Subcellular localization of ribosomes defines the location and capacity for protein synthesis. Methods for in vivo visualizing ribosomes in multicellular organisms are desirable in mechanistic investigations of the cell biology of ribosome dynamics. Here, we developed an approach using split GFP for tissue-specific visualization of ribosomes in Caenorhabditis elegans. Labeled ribosomes are detected as fluorescent puncta in the axons and synaptic terminals of specific neuron types, correlating with ribosome distribution at the ultrastructural level. We found that axonal ribosomes change localization during neuronal development and after axonal injury. By examining mutants affecting axonal trafficking and performing a forward genetic screen, we showed that the microtubule cytoskeleton and the JIP3 protein UNC-16 exert distinct effects on localization of axonal and somatic ribosomes. Our data demonstrate the utility of tissue-specific visualization of ribosomes in vivo, and provide insight into the mechanisms of active regulation of ribosome localization in neurons.
Databáze: OpenAIRE
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