Retinal Vascular Development and Pathologic Retinal Angiogenesis Are Not Impaired in Matrix Metalloproteinase-2 Deficient Mice
| Autor: | Sylvia Sarman, Ingeborg van der Ploeg, Anders Kvanta, Stefan Seregard |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Angiogenesis In situ hybridization Retinal Neovascularization Biology Matrix metalloproteinase Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Deficient mouse medicine Animals RNA Messenger In Situ Hybridization Mice Knockout Retina Reverse Transcriptase Polymerase Chain Reaction Retinal Vessels Dextrans Retinal Hypoxia (medical) Fluoresceins medicine.disease Sensory Systems Mice Inbred C57BL Oxygen Disease Models Animal Ophthalmology medicine.anatomical_structure Animals Newborn chemistry Matrix Metalloproteinase 2 Female medicine.symptom Retinopathy |
| Zdroj: | Current Eye Research. 30:259-267 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1080/02713680590923212 |
| Popis: | Earlier studies have suggested a role for metalloproteinase-2 (MMP-2) in retinal angiogenesis. To investigate this further, we have studied retinal vascular development and pathologic ischemia-induced retinal angiogenesis in MMP-2-deficient and wild-type mice.Vascular development of the retina was studied in retinal flatmounts, whereas pathologic retinal angiogenesis was analyzed in retinal flatmounts and on histologic sections using a model of ischemia-induced retinopathy. The time course of MMP-2 mRNA expression was determined by in situ hybridization and real-time polymerase chain reaction (PCR).Formation of the retinal vascular plexus was not significantly different in MMP-2-deficient mice as compared to wild-type mice. In ischemia-induced retinopathy, there was an increased formation of extraretinal neovascular tufts in the MMP-2-deficient mice (p0.05). MMP-2 mRNA expression did not correlate to either retinal vascular development or to ischemia-induced formation of extraretinal vascular tufts.The current data suggest that MMP-2 is not essential for either retinal vascular development or pathologic retinal neovascularization in the mouse. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |