Increased expression of annexin A1 is correlated with K-ras mutation in colorectal cancer
| Autor: | Qiang Wang, Can-Ping Ruan, Yan-Ping Sun, Han Chen, Ning Su, Wenchao Gao, Xin-Yun Xu |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Colorectal cancer medicine.medical_treatment Biology Gene mutation medicine.disease_cause General Biochemistry Genetics and Molecular Biology Metastasis Targeted therapy Proto-Oncogene Proteins p21(ras) Proto-Oncogene Proteins Gene expression medicine Humans RNA Messenger Annexin A1 Demography Mutation Predictive marker General Medicine medicine.disease Gene Expression Regulation Neoplastic Cancer research ras Proteins Female Colorectal Neoplasms |
| Zdroj: | The Tohoku journal of experimental medicine. 222(4) |
| ISSN: | 1349-3329 |
| Popis: | The activation of K-ras gene and expression of annexin A1 play an important role in colorectal tumorigenesis. We initiated this study to analyze the possible relationship between the annexin A1 expression and the K-ras mutation status in colorectal cancer. K-ras mutations are present in one fourth to one half of colorectal cancers. Annexin A1, a 37-kDa calcium- and phospholipid-binding protein, is over-expressed in colorectal cancers and may be involved in invasive tumor growth and metastasis. Here, we examined twenty paired specimens of colorectal cancer and adjacent normal tissues for K-ras mutations and annexin A1 expression. Sequencing analysis of codons 12 and 13 of K-ras revealed the presence of K-ras mutations in six colorectal cancer tissue specimens (30%). RT-PCR and immunoblotting studies further found that the expression levels of annexin A1 mRNA and protein were increased (2.9-fold and 1.7-fold, respectively) in colorectal cancers harboring K-ras codon 12 or codon 13 mutation compared with adjacent normal tissues (P < 0.05). In colorectal cancer tissues with wild-type K-ras, 12 (85.7%) specimens showed reduced expression of annexin A1 (0.48-fold and 0.81-fold, respectively). No significant association was found between K-ras mutations or annexin A1 over-expression and demographic or other clinicopathological parameters such as gender, differentiation or metastasis. However, a significant and positive correlation was identified between K-ras mutations and annexin A1 over-expression. Our findings indicate that annexin A1 could be implicated in colorectal cancer development and progression and could be of potential use as a predictive marker for guiding targeted therapy for colorectal cancer. |
| Databáze: | OpenAIRE |
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