Granular gland transcriptomes in stimulated amphibian skin secretions
| Autor: | Anthony J. Bjourson, Tianbao Chen, Pingfan Rao, Christopher Shaw, David F. Orr, Susan Farragher |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Amphibian
Bodily Secretions Proteome Neurokinin B Molecular Sequence Data Peptide Xenopus Proteins Bioinformatics Biochemistry Amphibian Proteins Amphibians Transcriptome biology.animal RNA Precursors Animals Secretion Amino Acid Sequence RNA Messenger Cloning Molecular Protein Precursors Molecular Biology Skin chemistry.chemical_classification Sequence Homology Amino Acid biology Proteins Vertebrate CDNA Library Construction Cell Biology Electric Stimulation Anti-Bacterial Agents Cell biology Alternative Splicing chemistry Amphibian Venoms Nucleic acid Peptides Research Article Antimicrobial Cationic Peptides |
| Zdroj: | Biochemical Journal. 371:125-130 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj20021343 |
| Popis: | Amphibian defensive skin secretions are complex, species-specific cocktails of biologically active molecules, including many uncharacterized peptides. The study of such secretions for novel peptide discovery is time-limited, as amphibians are in rapid global decline. While secretion proteome analysis is non-lethal, transcriptome analysis has until now required killing of specimens prior to skin dissection for cDNA library construction. Here we present the discovery that polyadenylated mRNAs encoding dermal granular gland peptides are present in defensive skin secretions, stabilized by endogenous nucleic acid-binding amphipathic peptides. Thus parallel secretory proteome and transcriptome analyses can be performed without killing the specimen in this model amphibian system—a finding that has important implications in conservation of biodiversity within this threatened vertebrate taxon and whose mechanistics may have broader implications in biomolecular science. |
| Databáze: | OpenAIRE |
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