The Caenorhabditis elegans JIP3 protein UNC-16 functions as an adaptor to link kinesin-1 with cytoplasmic dynein
| Autor: | Bikash C. Choudhary, Makoto Arimoto, Sandhya P. Koushika, Naoki Hisamoto, Chris Li, Kunihiro Matsumoto |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Cytoplasmic Dyneins
Nexin Microinjections Dynein Presynaptic Terminals Kinesins macromolecular substances Transfection Animals Genetically Modified Microtubule Chlorocebus aethiops medicine Animals Immunoprecipitation Axon Caenorhabditis elegans Caenorhabditis elegans Proteins Adaptor Proteins Signal Transducing biology General Neuroscience Dyneins Membrane Proteins Biological Transport Articles biology.organism_classification Cell biology Luminescent Proteins Protein Transport medicine.anatomical_structure Gene Expression Regulation COS Cells biology.protein Axoplasmic transport Dynactin Kinesin Mechanoreceptors Protein Binding |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(6) |
| ISSN: | 1529-2401 |
| Popis: | Kinesin-1 is a microtubule plus-end-directed motor that transports various cargos along the axon. Previous studies have elucidated the physical and genetic interactions between kinesin-1 and cytoplasmic dynein, a microtubule minus-end-directed motor, in neuronal cells. However, the physiological importance of kinesin-1 in the dynein-dependent retrograde transport of cargo molecules remains obscure. Here, we show thatCaenorhabditis eleganskinesin-1 forms a complex with dynein via its interaction with UNC-16, which binds to the dynein light intermediate (DLI) chain. Both kinesin-1 and UNC-16 are required for localization of DLI-1 at the plus ends of nerve process microtubules. In addition, retrograde transport of APL-1 depends on kinesin-1, UNC-16, and dynein. These results suggest that kinesin-1 mediates the anterograde transport of dynein using UNC-16 as a scaffold and that dynein in turn mediates the retrograde transport of cargo moleculesin vivo. Thus, UNC-16 functions as an adaptor for kinesin-1-mediated transport of dynein. |
| Databáze: | OpenAIRE |
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