Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica
| Autor: | Tod Stewart, Arthur Hinton, Deana R. Jones, Paula J. Fedorka-Cray, Jo Ann S. Van Kessel, Richard K. Gast, Roxana Sanchez-Ingunza, Kim Ingram, Jonathan G. Frye, Jean Guard, Karen Liljebjelke, Cesar A. Morales, Charlene R. Jackson |
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| Rok vydání: | 2012 |
| Předmět: |
Serotype
DNA Bacterial Single-nucleotide polymorphism Microbiology Ribotyping Intergenic region single nucleotide polymorphism Genetics Animals Humans Serotyping Molecular Biology Gene Oligonucleotide Array Sequence Analysis Salmonella Infections Animal biology Salmonella enterica Ribosomal RNA biology.organism_classification Microarray Analysis Molecular biology Research Letters food safety Salmonella Infections DNA Intergenic epidemiology DNA microarray Sugar Alcohol Dehydrogenases |
| Zdroj: | Fems Microbiology Letters |
| ISSN: | 1574-6968 |
| Popis: | Two DNA-based methods were compared for the ability to assign serotype to 139 isolates of Salmonella enterica ssp. I. Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB. A DNA microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. Serotype was assigned for 128 (92.1%) of submissions by the two DNA methods. ISR detected mixtures of serotypes within single colonies and it cost substantially less than Kauffmann–White serotyping and DNA microarray hybridization. Decreasing the cost of serotyping S. enterica while maintaining reliability may encourage routine testing and research. |
| Databáze: | OpenAIRE |
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