Assembly of Multiple CotC Forms into the Bacillus subtilis Spore Coat
| Autor: | Rita Zilhão, Giuseppina Cangiano, Maurilio De Felice, Adriano O. Henriques, Rachele Isticato, Sofia Nolasco, Giovanni Esposito, Ezio Ricca |
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| Přispěvatelé: | Isticato, Rachele, Esposito, G, Zilhão, R, Nolasco, S, Cangiano, Giuseppina, DE FELICE, Maurilio, Henriques, Ao, Ricca, Ezio |
| Rok vydání: | 2004 |
| Předmět: |
Spores
Bacterial Bacillaceae biology Molecular Sequence Data fungi Mutant Meeting Presentations Bacillus subtilis Compartment (chemistry) biology.organism_classification Microbiology Bacillales Spore Molecular Weight protein complexe Bacterial Proteins Biochemistry Two-Hybrid System Techniques Escherichia coli Amino Acid Sequence Molecular Biology Peptide sequence spore coat Bacteria |
| Zdroj: | Journal of Bacteriology. 186:1129-1135 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.186.4.1129-1135.2004 |
| Popis: | We report evidence that the CotC polypeptide, a previously identified component of the Bacillus subtilis spore coat, is assembled into at least four distinct forms. Two of these, having molecular masses of 12 and 21 kDa, appeared 8 h after the onset of sporulation and were probably assembled on the forming spore immediately after their synthesis, since no accumulation of either of them was detected in the mother cell compartment, where their synthesis occurs. The other two components, 12.5 and 30 kDa, were generated 2 h later and were probably the products of posttranslational modifications of the two early forms occurring directly on the coat surface during spore maturation. None of the CotC forms was found either on the spore coat or in the mother cell compartment of a cotH mutant. This indicates that CotH serves a dual role of stabilizing the early forms of CotC and promoting the assembly of both early and late forms on the spore surface. |
| Databáze: | OpenAIRE |
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