Conservation of the central proline-rich (PxxP) motifs of human immunodeficiency virus type 1 Nef protein during the disease progression in two hemophiliac patients
| Autor: | Takashi Morishima, Takashi Okamoto, Kaori Asamitsu, Hideaki Tsuchie, Takashi Kurimura |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
nef Protein Conformation Sequence analysis viruses Molecular Sequence Data Biophysics Down-Regulation HIV Infections SH3 ligand Viral quasispecies Hemophilia A Major histocompatibility complex Biochemistry Gene Products nef Conserved sequence Evolution Molecular Protein structure Structural Biology Molecular evolution Genetics Humans Amino Acid Sequence nef Gene Products Human Immunodeficiency Virus Molecular Biology Peptide sequence Gene Conserved Sequence Phylogeny Sequence Homology Amino Acid biology Histocompatibility Antigens Class I Genetic Variation virus diseases Cell Biology Virology AIDS CD4 Antigens Disease Progression HIV-1 biology.protein Proline-Rich Protein Domains Peptides Sequence Analysis |
| Zdroj: | FEBS Letters. 459:399-404 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(99)01288-0 |
| Popis: | The nef gene is considered to play a crucial role in the development of acquired immunodeficiency syndrome (AIDS). In this study, we analyzed the sequence of nef quasispecies obtained from replication-competent HIV-1 isolates from two Japanese hemophiliac patients infected with HIV-1. At least 10 nef clones were isolated at each time point and a total of 75 individual nef quasispecies were sequenced. We observed a gradual increase in genetic diversity of the nef gene over time. Among the various functional regions of Nef protein, myristoylation site and the central PXXP (SH3 ligand) motifs were well conserved. The scattered regions responsible for downregulation of CD4 and class I MHC were also conserved. These data suggest that these functions of Nef may be involved throughout the disease process. |
| Databáze: | OpenAIRE |
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