Ecotopic over-expression of PoCHS from Paeonia ostii altered the fatty acids composition and content in Arabidopsis thaliana
| Autor: | Qing Hao, Li-Ping Peng, Wen-Yuan Huang, Xiao-Feng Ma, Ning-Ning Tong, Mo Chen, Qing-Yan Shu, Si-Qi Men, Xi-Ruo Wang, Zheng-An Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chalcone synthase Physiology Arabidopsis Plant Science Paeonia 01 natural sciences 03 medical and health sciences Genetics Arabidopsis thaliana Gene Unsaturated fatty acid chemistry.chemical_classification biology Fatty Acids Cell Biology General Medicine biology.organism_classification Amino acid Biosynthetic Pathways Paeonia ostii 030104 developmental biology Enzyme chemistry Biochemistry Seeds biology.protein 010606 plant biology & botany |
| Zdroj: | Physiologia plantarumREFERENCES. 172(1) |
| ISSN: | 1399-3054 |
| Popis: | Chalcone synthase (CHS) is the key enzyme in the flavonoid biosynthetic pathway and has been studied in many plants, but the function of the CHS gene has not been well characterized in Paeonia ostii. In this study, we obtained a CHS homologue gene from P. ostii, which possessed the putative conserved amino acids of chalcone synthase by multiple alignment analysis and demonstrated the highest expression in developing seeds. In vitro assays of the recombinant PoCHS protein confirmed enzymatic activity using malonyl-CoA and 4-coumaroyl-CoA as substrates, and the optimal pH and reaction temperature were 7.5 and 40°C, respectively. Furthermore, ectopic over-expression of PoCHS in Arabidopsis up-regulated the expression levels of genes involved in seed development (ABI), glycolysis (PKp2, PDH-E1a, and SUS2/3), and especially fatty acid biosynthesis (BCCP2, CAC2, CDS2, FatA and FAD3). This resulted in an increased unsaturated fatty acid content, especially α-linolenic acid, in transgenic Arabidopsis seeds. In this study, we examined the functions of CHS homologue in P. ostii and demonstrated its new function in seed fatty acid biosynthesis. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text