Horizontal gene transfer of a syp homolog contributes to the virulence of Burkholderia glumae
| Autor: | Ayizekeranmu Yiming, Zhouqi Cui, Iftikhar Ahmad, Yan Wu, Peihong Wang, Bo Zhu, Gongyou Chen, Luo-yi Fu, Sai Wang, Wenhan Nie, Hong-yu Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Genetics
Ecology biology Agriculture (General) Pseudomonas Mutant Wild type Virulence burkholderia glumae Pathogenic bacteria Plant Science biology.organism_classification medicine.disease_cause Biochemistry syringopeptin synthetase S1-972 Food Animals Horizontal gene transfer medicine Burkholderia glumae horizontal gene transfer Animal Science and Zoology Agronomy and Crop Science Gene Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 20, Iss 12, Pp 3222-3229 (2021) |
| ISSN: | 2095-3119 |
| Popis: | Horizontal gene transfer (HGT) has been proved a major driving force in prokaryotic evolution. However, the molecular functions of these transferred genes in pathogenic bacteria especially plant pathogenic bacteria are still not fully investigated. In this study, the whole-genome in silico analysis was performed and found a syringopeptin synthetase (syp) homolog in Burkholderia glumae, which can cause bacterial panicle blight in rice, was predicted to be horizontally transferred from Pseudomonas ancestor with solid confidence by phylogenetic analysis. The comprehensive molecular experiments were performed to study the potential role of this gene in B. glumae. Inoculation of rice panicles with the syp mutant resulted in 60% lower disease index compared with the wild type (WT) parent strain, suggesting the requirement of syp for the full virulence of B. glumae. Chromatography analysis of exudates from B. glumae showed suppression of synthesis of metabolites analogous to syringopeptin in the mutants. All these data raise the possibility of HGT phenomenon in shaping the virulence and adaptation of B. glumae over evolutionary time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect