Burkholderia perseverans sp. nov., a bacterium isolated from the Restinga ecosystem, is a producer of volatile and diffusible compounds that inhibit plant pathogens
| Autor: | Jackeline Pereira Andrade, Aristóteles Góes-Neto, Harisson Guimarães de Souza, Phellippe Arthur Santos Marbach, Evelien De Canck, Larissa Carvalho Ferreira, Eduardo Gross, Jorge Teodoro de Souza, Peter Vandamme, Margo Cnockaert, Charlotte Peeters, Anneleen D. Wieme |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial Phytophthora Burkholderia gladioli Burkholderia Microbiology Species Specificity RNA Ribosomal 16S Mycology Antibiosis Media Technology Burkholderia glumae Environmental Microbiology - Research Paper Ecosystem Phospholipids Phylogeny Volatile Organic Compounds biology Strain (chemistry) food and beverages biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Plant Leaves Aspergillus Agaricales Brazil Bacteria Burkholderia plantarii |
| Zdroj: | Braz J Microbiol |
| ISSN: | 1678-4405 1517-8382 |
| DOI: | 10.1007/s42770-021-00560-w |
| Popis: | Gram-negative, aerobic, rod-shaped, non-spore-forming, motile bacteria, designated CBAS 719( T), CBAS 732 and CBAS 720 were isolated from leaf litter samples, collected in Espírito Santo State, Brazil, in 2008. Sequences of the 16S rRNA, gyrB, lepA and recA genes showed that these strains grouped with Burkholderia plantarii LMG 9035( T), Burkholderia gladioli LMG 2216( T) and Burkholderia glumae LMG 2196( T) in a clade of phytopathogenic Burkholderia species. Digital DNA-DNA hybridization experiments and ANI analyses demonstrated that strain CBAS 719( T) represents a novel species in this lineage that is very closely related with B. plantarii. The genome sequence of the type strain is 7.57 Mbp and its G + C content is 69.01 mol%. The absence of growth on TSA medium supplemented with 3% (w/v) NaCl, citrate assimilation, β-galactosidase (PNPG) activity, and of lipase C14 activity differentiated strain CBAS 719( T) from B. plantarii LMG 9035( T), its nearest phylogenetic neighbor. Its predominant fatty acid components were C(16:0), C(18:1) ω7c, cyclo-C(17:0) and summed feature 3 (C(16:1) ω7c and/or C(15:0) iso 2-OH). Based on these genotypic and phenotypic characteristics, the strains CBAS 719( T), CBAS 732 and CBAS 720 are classified in a novel Burkholderia species, for which the name Burkholderia perseverans sp. nov. is proposed. The type strain is CBAS 719( T) (= LMG 31557( T) = INN12(T)). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-021-00560-w. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink