Characterization and genetic mapping of fructose phosphotransferase mutations in Pseudomonas aeruginosa
| Autor: | P V Phibbs, R A Roehl |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Genetic Linkage
Mutant Dehydrogenase Fructose macromolecular substances Biology medicine.disease_cause Microbiology Phosphotransferase chemistry.chemical_compound Plasmid Transduction Genetic otorhinolaryngologic diseases medicine Phosphoenolpyruvate Sugar Phosphotransferase System Molecular Biology Gene Pseudomonas aeruginosa Chromosome Mapping PEP group translocation Molecular biology carbohydrates (lipids) stomatognathic diseases Biochemistry chemistry Genes Bacterial Conjugation Genetic Mutation bacteria Research Article |
| Zdroj: | Journal of Bacteriology. 149:897-905 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.149.3.897-905.1982 |
| Popis: | Pseudomonas aeruginosa transports and phosphorylates fructose via a phosphoenolpyruvate-dependent fructose phosphotransferase system (PTS). Mutant strains deficient in both PTS activity and glucose-6-phosphate dehydrogenase activity were isolated and were used to select mannitol-utilizing revertant strains singly deficient in PTS activity. These mutants were unable to utilize fructose as a carbon source and failed to accumulate exogenously provided [14C]fructose, and crude cell extracts lacked phosphoenolpyruvate-dependent fructose PTS activity. Thus, the PTS was essential for the uptake and utilization of exogenously provided fructose by P. aeruginosa. Mutations at a locus designated pts, which resulted in a loss of PTS activity, exhibited 57% linkage to argF at 55 min on the chromosome in plasmid R68.45-mediated conjugational crosses. The pts mutations in four independently isolated mutant strains exhibited from 11 to 20% linkage to argF, and one of these mutations exhibited 3% linkage to lys-9015 in phage F116L-mediated transductional crosses. |
| Databáze: | OpenAIRE |
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