Formulating erythropoietin-loaded sustained-release PLGA microspheres without protein aggregation
| Autor: | Jingle Chen, Yan Geng, Mu He, Tuo Jin, Weien Yuan, Fei Wu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Biological Availability Pharmaceutical Science Polyethylene glycol Dosage form Polyethylene Glycols Mice chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer X-Ray Diffraction hemic and lymphatic diseases Polymer chemistry PEG ratio Animals Humans Erythropoiesis Lactic Acid Microparticle Erythropoietin Mice Inbred BALB C Chromatography technology industry and agriculture Dextrans Microspheres Recombinant Proteins Solvent PLGA Dextran chemistry Delayed-Action Preparations Antibody Formation Emulsion Erythrocyte Count Polyglycolic Acid |
| Zdroj: | Journal of Controlled Release. 130:259-265 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2008.06.011 |
| Popis: | We report a simple method to microencapsulate erythropoietin (EPO, a protein easily denatured and antigenized by contact with water-organic solvent interfaces) into poly(lactic-co-glycolic acid) (PLGA) microspheres with minimal aggregation. This formulation process involved an aqueous-aqueous emulsion formed at reduced temperature. EPO was first dissolved in water together with dextran (MW=70,000) and polyethylene glycol (MW=8000), followed by a freezing process during which dextran separated out as the dispersed phase with EPO partitioned in preferentially. The frozen sample was then lyophilized to powder and washed with dichloromethane to remove the PEG continuous phase. Once loaded in the polysaccharide particles, 1-4 microm in diameter, EPO gained resistance to organic solvents and was encapsulated into PLGA microspheres without significant aggregation ( |
| Databáze: | OpenAIRE |
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