Cytometric catheter for neurosurgical applications
| Autor: | George T. Gillies, Helen L. Fillmore, William C. Broaddus, Stephen W. Allison, Boyd M. Evans, Rachel L Dyer |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.diagnostic_test
business.industry Cell Central nervous system Biomedical Engineering Cell- and Tissue-Based Therapy General Medicine Equipment Design Neural stem cell Neurosurgical Procedures Flow cytometry Catheterization Cell therapy Catheter medicine.anatomical_structure Port (medical) medicine Animals Humans Stem cell business Biomedical engineering |
| Zdroj: | Journal of medical engineeringtechnology. 34(4) |
| ISSN: | 1464-522X |
| Popis: | Implantation of neural progenitor cells into the central nervous system has attracted strong interest for treatment of a variety of pathologies. The replacement of dopamine-producing neural cells in the brain appears promising for the treatment of patients affected by Parkinson's disease. Previous studies of cell replacement strategies have shown that less than 10% of implanted cells were viable 24–48 hours following implantation. We present the design of an instrumented cell-delivery catheter that has been developed to facilitate the quantification of the cells delivered and determination of viability. The catheter uses a fibre optic probe to perform fluorescence-based cytometric measurements on cells exiting the port at the catheter tip. Results of fluorescence testing data are presented and show that the device can characterize the quantity of cell densities ranging from 60 000 to 600 000 cells ml−1 with a coefficient of determination of 0.93 (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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