Photoactivatable drugs for nicotinic optopharmacology
| Autor: | Yong Wang, J. Michael McIntosh, Nicholas Bannon, Anis Contractor, Veronica J. Kim, Luke D. Lavis, Guiqing Zhao, Kyle R. Gee, Yevgenia Kozorovitskiy, John J. Macklin, Xiao Tao Jin, Matthew C. Arvin, Can Peng, Sambashiva Banala, Henry A. Lester, John Marshall, Ryan M. Drenan, David L. Wokosin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nicotine General method Receptors Nicotinic Biochemistry 03 medical and health sciences Mice medicine Animals Molecular Biology Acetylcholine receptor Microscopy Confocal Chemistry Brain Cell Biology Photochemical Processes Immunohistochemistry Red shift 030104 developmental biology Nicotinic agonist Spectrometry Fluorescence Biophysics Calcium Spectrophotometry Ultraviolet Biotechnology medicine.drug |
| Zdroj: | Nature methods. 15(5) |
| ISSN: | 1548-7105 |
| Popis: | Photoactivatable pharmacological agents have revolutionized neuroscience, but the palette of available compounds is limited. We describe a general method for caging tertiary amines by using a stable quaternary ammonium linkage that elicits a red shift in the activation wavelength. We prepared a photoactivatable nicotine (PA-Nic), uncageable via one- or two-photon excitation, that is useful to study nicotinic acetylcholine receptors (nAChRs) in different experimental preparations and spatiotemporal scales. |
| Databáze: | OpenAIRE |
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