A Modular Platform for Cytocompatible Hydrogels with Tailored Mechanical Properties Based on Monolithic Matrices and Particulate Building Blocks
| Autor: | Lea Andrée, Pascal Bertsch, Rong Wang, Malin Becker, Jeroen Leijten, Peter Fischer, Fang Yang, Sander C. G. Leeuwenburgh |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Biomacromolecules, 24 (6) Biomacromolecules, 24, 2755-2765 Biomacromolecules, 24, 6, pp. 2755-2765 |
| ISSN: | 1525-7797 1526-4602 |
| Popis: | We establish a versatilehydrogel platform based on modularbuildingblocks that allows the design of hydrogels with tailored physicalarchitecture and mechanical properties. We demonstrate its versatilityby assembling (i) a fully monolithic gelatin methacryloyl (Gel-MA)hydrogel, (ii) a hybrid hydrogel composed of 1:1 Gel-MA and gelatinnanoparticles, and (iii) a fully particulate hydrogel based on methacryloyl-modifiedgelatin nanoparticles. The hydrogels were formulated to exhibit thesame solid content and comparable storage modulus but different stiffnessand viscoelastic stress relaxation. The incorporation of particlesresulted in softer hydrogels with enhanced stress relaxation. Murineosteoblastic cells cultured in two-dimensional (2D) on hydrogels showedproliferation and metabolic activity comparable to established collagenhydrogels. Furthermore, the osteoblastic cells showed a trend of increasedcell numbers, cell expansion, and more defined protrusions on stifferhydrogels. Hence, modular assembly allows the design of hydrogelswith tailored mechanical properties and the potential to alter cellbehavior. Biomacromolecules, 24 (6) ISSN:1525-7797 ISSN:1526-4602 |
| Databáze: | OpenAIRE |
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