Selective procedures for detecting femtomole quantities of tuberculostearic acid in serum and cerebrospinal fluid by frequency-pulsed electron capture gas-liquid chromatography
| Autor: | M I Daneshvar, D M Fast, R C Good, J B Brooks |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Microbiology (medical)
Chromatography Gas Chromatography Chemistry Electron capture Capillary action Extraction (chemistry) Tuberculostearic acid Sorption medicine.disease Tuberculous meningitis Electron capture detector chemistry.chemical_compound Biochemistry Tuberculosis Meningeal medicine Humans lipids (amino acids peptides and proteins) Gas chromatography Stearic Acids Research Article |
| Zdroj: | Journal of Clinical Microbiology. 25:1201-1206 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.25.7.1201-1206.1987 |
| Popis: | Conditions are described for the detection of tuberculostearic acid (10-methyloctadecanoate; C18 X CH3) in cerebrospinal fluid and serum of patients with tuberculous meningitis. C18 X CH3 was found in both the cerebrospinal fluid and serum of patients with tuberculous meningitis at concentrations of 25 to 50 fmol (10(-15) mol). The necessary specificity and sensitivity for detection of C18 X CH3 were obtained by extraction under acid conditions with organic solvent, specific functional group esterification with trichloroethanol, cleanup with disposable reverse-phase sorption chromatography columns, analysis on high-resolution polar and nonpolar capillary columns, and detection by a frequency-pulsed electron capture detector. Use of an IBM 9000 computer equipped with CAP software significantly aided comparison between known C18 X CH3 standards and C18 X CH3 in clinical specimens. Scale expansion and attenuation changes were the major contributions obtained by use of the computer. The data indicate that detection of C18 X CH3 by frequency-pulsed electron capture gas-liquid chromatography may be a valuable aid for early detection of tuberculous meningitis. |
| Databáze: | OpenAIRE |
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