Genetic variability in G2 and F2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure
| Autor: | Sandra Elisabete Vieira, Viviane Fongaro Botosso, Hildener Nogueira Lima, Edison Luiz Durigon, Klaus Eberhard Stewien, Patrícia Alves Ramos Bosso, Claudia T. P. Moraes, Danielle Bruna Leal Oliveira, Angélica Cristine de Almeida Campos, Alfredo Elias Gilio |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine Genes Viral lcsh:RC955-962 Short Communication HRSV lcsh:QR1-502 Mothers Respiratory Syncytial Virus Infections Viral Plaque Assay Antibodies Viral medicine.disease_cause lcsh:Microbiology Virus genetic variability Genetic variation medicine Humans Genetic variability Child Gene immune selection pressure Genetics Virus quantification Mutation Base Sequence biology Genetic Variation Nasal Lavage Fluid Virology Respiratory Syncytial Virus Human biology.protein Antibody Synonymous substitution |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 110, Issue: 1, Pages: 138-141, Published: 03 FEB 2015 Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz., Vol 110, Iss 1, Pp 138-141 (2015) |
| ISSN: | 0074-0276 |
| Popis: | Human respiratory syncytial virus (HRSV) is an important respiratory pathogens among children between zero-five years old. Host immunity and viral genetic variability are important factors that can make vaccine production difficult. In this work, differences between biological clones of HRSV were detected in clinical samples in the absence and presence of serum collected from children in the convalescent phase of the illness and from their biological mothers. Viral clones were selected by plaque assay in the absence and presence of serum and nucleotide sequences of the G2 and F2 genes of HRSV biological clones were compared. One non-synonymous mutation was found in the F gene (Ile5Asn) in one clone of an HRSV-B sample and one non-synonymous mutation was found in the G gene (Ser291Pro) in four clones of the same HRSV-B sample. Only one of these clones was obtained after treatment with the child's serum. In addition, some synonymous mutations were determined in two clones of the HRSV-A samples. In conclusion, it is possible that minor sequences could be selected by host antibodies contributing to the HRSV evolutionary process, hampering the development of an effective vaccine, since we verify the same codon alteration in absence and presence of human sera in individual clones of BR-85 sample. |
| Databáze: | OpenAIRE |
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