LHRH-conjugated, PEGylated, poly-lactide-co-glycolide nanocapsules for targeted delivery of combinational chemotherapeutic drugs Docetaxel and Quercetin for prostate cancer
| Autor: | Ajinkya A. Shitole, Anand P. Khandwekar, Neeti Sharma, Santosh S. Koratkar, Prabhanjan S. Giram, Meghna M. Baruah, Baijayantimala Garnaik |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Materials science Bioengineering Antineoplastic Agents 02 engineering and technology Docetaxel Pharmacology 010402 general chemistry behavioral disciplines and activities 01 natural sciences Nanocapsules Polyethylene Glycols Biomaterials Dioxanes Gonadotropin-Releasing Hormone chemistry.chemical_compound In vivo Cell Line Tumor mental disorders LNCaP PEG ratio medicine Humans Viability assay Drug Carriers Prostatic Neoplasms 021001 nanoscience & nanotechnology Controlled release 0104 chemical sciences PLGA chemistry Mechanics of Materials Nanoparticles Quercetin 0210 nano-technology medicine.drug |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 114 |
| ISSN: | 1873-0191 |
| Popis: | One of the major challenges in effective cancer chemotherapy is the severe systemic cytotoxicities of anticancer drugs on healthy tissues. The present study reports chemically modified polymeric nanocapsules (NCs) encapsulating combination of chemotherapeutic drugs Docetaxel (DTX) and Quercetin (QU) for its active targeting to prostate cancer (PCa). The active targeting was achieved by conjugating Luteinizing-hormone-releasing hormone (LHRH) ligand to poly-lactide-co-glycolide (PLGA) using polyethylene glycol (PEG) as a spacer. The structure of the conjugates was characterized and confirmed using 1H NMR and ATR-FTIR. The drug encapsulated NCs showed a homogenous size distribution with their size ranging between 120 and 150 nm, and exhibited a negative zeta potential in the range of −20 to −40 mV. The in vitro release studies highlighted the sustained drug release pattern from the respective NCs; while the PEG coating to polymeric NCs provided serum stability to the NCs. The in vitro biological evaluation of the NCs was conducted using PC-3 and LNCaP cell lines. The results of the cellular uptake studies showed a significantly higher untake of the LHRH targeted NCs, while the LHRH-targeted-PEGylated DTX: QU NCs exhibited higher caspase-3 activity. The cell viability assay results showed the enhanced cell inhibition activity of the combinatorial DTX: QU when compared to individual DTX. Further, higher cell cytotoxicity was achieved by LHRH-targeted DTX: QU NCs as compared to their free-form or non-targeted NCs. Finally, the results of in vivo tumor localization and in vivo antitumor activity studies complimented and upheld the in vitro results, demonstrating the beneficial role of PLGA-PEG-LHRH NCs encapsulating combination of DTX and QU in combating prostate cancer (PCa). |
| Databáze: | OpenAIRE |
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