Assignment of the human prohibition gene (PHB) to chromosome 17 and identification of a DNA polymorphism
| Autor: | Mark J. Nuell, Garrison A. Owens, Varda Friedman, John J. White, Roger L. Eddy, Cynthia C. Morton, Carla M. Wood, David H. Ledbetter, David B. Danner, Thomas B. Shows, J.Keith McClung, David A. Stewart |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Tumor suppressor gene Biology chemistry.chemical_compound Gene mapping Prohibitins Genetics Humans Gene Metaphase Chromosome Mapping Proteins Karyotype Molecular biology Chromosome Banding Pedigree Repressor Proteins Chromosome 17 (human) Blotting Southern chemistry Female Restriction fragment length polymorphism Polymorphism Restriction Fragment Length DNA Chromosomes Human Pair 17 |
| Zdroj: | Genomics. 11:228-230 |
| ISSN: | 0888-7543 |
| Popis: | Prohibition is a recently identified antiproliferative protein whose exact role in the cell is under investigation. To determine the human chromosomal location of the prohibition gene (PHB) and whether this site corresponds to that of any suspected tumor suppressor gene, we have analyzed DNA from three sources by hybridization analysis: mouse--human hybrid cell lines, hybrid cell lines containing portions of human chromosomes, and human metaphase chromosomes in situ. All three techniques confirm a location in the region 17q21-q22, a region genetically linked to early-onset human breast cancer. Further analysis will be required to establish the significance of this relationship; Southern hybridizations show a polymorphic EcoRI site that may be useful for this purpose. |
| Databáze: | OpenAIRE |
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