The synergetic effect of edaravone and borneol in the rat model of ischemic stroke
| Autor: | Yao Hua, Chun-Xia Luo, Li-Yan Gao, Dong-Ya Zhu, Zhang Zhengping, Gao-Yong Liao, Ying Tang, Shi-Bao Yang, Hai-Yin Wu, Qi-Gang Zhou, Wei-Xiang Sun |
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| Rok vydání: | 2014 |
| Předmět: |
Programmed cell death
Cell Survival Interleukin-1beta Ischemia Anti-Inflammatory Agents Pharmacology Borneol Brain Ischemia Rats Sprague-Dawley chemistry.chemical_compound Edaravone medicine Animals Maze Learning ED50 Cells Cultured Neurons Camphanes biology Chemistry Tumor Necrosis Factor-alpha Brain Drug Synergism Free Radical Scavengers Free radical scavenger medicine.disease Coculture Techniques Nitric oxide synthase Stroke Disease Models Animal Cyclooxygenase 2 Anesthesia biology.protein Tumor necrosis factor alpha Drug Therapy Combination Neuroglia Antipyrine |
| Zdroj: | European journal of pharmacology. 740 |
| ISSN: | 1879-0712 |
| Popis: | Free radical production contributes to the early ischemic response and the neuroinflammatory response to injury initiates the second wave of cell death following ischemic stroke. Edaravone is a free radical scavenger, and borneol has shown anti-inflammatory effect. We investigated the synergistic effect of these two drugs in the rat model of transient cerebral ischemia. Edaravone scavenged OH, NO and ONOO─ concentration-dependently, and borneol inhibited ischemia/reperfusion-induced tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) expressions. In the rat model of transient cerebral ischemia and reperfusion, the combination of edaravone and borneol significantly ameliorated ischemic damage with an optimal proportion of 4:1. Emax (% inhibition) of edaravone, borneol and two drugs in combination was 55.7%, 65.8% and 74.3% respectively. ED50 of edaravone and borneol was 7.17 and 0.36 mg/kg respectively. When two drugs in combination, ED50 was 0.484 mg/kg, in which edaravone was 0.387 mg/kg (ineffective dose) and borneol was 0.097 mg/kg (ineffective dose). Combination index (CI) |
| Databáze: | OpenAIRE |
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