Parvovirus B19 seroprevalence, viral load, and genotype characterization in volunteer blood donors from southern Brazil

Autor: Virginie Sauvage, Svetoslav Nanev Slavov, Evandra Straza Rodrigues, Cristiane Fração Diefenbach, Simone Kashima, Syria Laperche, Dimas Tadeu Covas, Valérie Caro, Ana Maria Zimmermann
Přispěvatelé: Universidade de São Paulo = University of São Paulo (USP), Institut National de la Transfusion Sanguine [Paris] (INTS), Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur [Paris] (IP), Universidade Federal de Santa Maria = Federal University of Santa Maria [Santa Maria, RS, Brazil] (UFSM), Hospital 'Dr. Astrogildo de Azevedo' [Santa Maria, Brazil] (HCAA), This study was supported by Fundação de Amparo e Pesquisa do Estado de São Paulo‐FAPESP, Brazil (grant nos. 2009/16623‐1, CTC‐2013/081352, 2017/23205‐8, and INCTC‐FAPESP‐2014/50947‐7), Conselho Nacional do Desenvolvimento Científico e Tecnológico, Brazil (INCTC‐465539/2014‐9 and 455503/2014‐1), and FINEP, Brazil (245/2016)., We are grateful to all blood donors who donated their samples for realization of this study. We also thank to Johanna Gomez form INTS, Paris, France for performing metagenomic deep‐sequencing experiments and Dr. Mathias Vandenbogaert from the Pole for Genotyping of Pathogens, Laboratory for Urgent Response to Biological Threats, Institut Pasteur, Paris, France for performing the data analysis in metagenomic samples. We would like to thank Sandra Navarro Bresciani for the excellent artwork., Universidade de São Paulo (USP), Institut Pasteur [Paris], Universidade Federal de Santa Maria (UFSM)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Blood transfusion
medicine.medical_treatment
real-time polymerase chain reaction
Antibodies
Viral
MESH: Genotype
0302 clinical medicine
Seroepidemiologic Studies
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Genotype
Parvovirus B19
Human
Prevalence
Medicine
030212 general & internal medicine
MESH: Immunoglobulin G
MESH: Aged
MESH: Middle Aged
biology
seroprevalence
Middle Aged
Viral Load
MESH: Immunoglobulin M
3. Good health
Infectious Diseases
Real-time polymerase chain reaction
MESH: Young Adult
blood donors
Female
030211 gastroenterology & hepatology
MESH: Viral Load
Viral load
Brazil
Adult
MESH: Parvovirus B19
Human
Adolescent
MESH: Parvoviridae Infections
Parvoviridae Infections
Young Adult
03 medical and health sciences
genotypes
Virology
Humans
Seroprevalence
POLIMERIZAÇÃO
Adverse effect
MESH: Prevalence
Aged
Retrospective Studies
MESH: Adolescent
MESH: Seroepidemiologic Studies
MESH: Humans
parvovirus B19
Parvovirus
business.industry
MESH: Blood Donors
Retrospective cohort study
MESH: Adult
MESH: Retrospective Studies
biology.organism_classification
MESH: Male
MESH: DNA
Viral
Immunoglobulin M
Immunoglobulin G
DNA
Viral
business
MESH: Brazil
MESH: Female
MESH: Antibodies
Viral
Zdroj: Journal of Medical Virology
Journal of Medical Virology, 2019, 91 (7), pp.1224-1231. ⟨10.1002/jmv.25453⟩
Journal of Medical Virology, Wiley-Blackwell, 2019, 91 (7), pp.1224-1231. ⟨10.1002/jmv.25453⟩
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 0146-6615
1096-9071
DOI: 10.1002/jmv.25453⟩
Popis: International audience; Usually transmitted via respiratory droplets, parvovirus B19 (B19V) can also be acquired by blood transfusion especially because of viral persistence, resistance to blood treatment procedures, and high viral load during the early infection phase. This is particularly problematic in immunocompromised or anemic patients where the infection can have a severe outcome. As B19V DNA was detected in blood donations from South Brazil during a viral metagenomic survey performed by Next-Generation Sequencing, the objective of this retrospective study was to evaluate the seroprevalence, B19V DNA presence and circulating genotypes in a Hospital Blood Transfusion Service in Santa Maria city in South Brazil (Rio Grande do Sul state). Among 480 volunteer blood donors, 53.9% (n = 258 of 479) were anti-B19V IgG-positive, and 9 (1.9%) plasma samples presented B19V DNA. In almost all cases (n = 7 of 9, 77.8%), B19V DNA load was accompanied by the presence of anti-B19V IgG suggesting a persistent infection. The sequencing of the strains demonstrated that all belong to genotype 1 which is the most prevalent worldwide. The analysis of the recipient information of the positive for B19V DNA units revealed no related posttransfusion adverse effects. Our results demonstrate for the first time, B19V seroprevalence, viral load, and genotypes among blood donors from South Brazil and give a light for the circulation and impact of this B19V in this part of the country.
Databáze: OpenAIRE
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