Loading of collagen-heparan sulfate matrices with bFGF promotes angiogenesis and tissue generation in rats

Autor: A.H.M.S.M. van Kuppevelt, Theo Hafmans, M.J.A. van Luyn, P.B. van Wachem, J.H. Veerkamp, J.S. Pieper, Linda A. Brouwer
Přispěvatelé: Faculteit Medische Wetenschappen/UMCG, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Rok vydání: 2002
Předmět:
collagen
Materials science
Chemical Phenomena
Angiogenesis
medicine.medical_treatment
Basic fibroblast growth factor
DIVERSITY
Biomedical Engineering
Neovascularization
Physiologic
Fibroblast growth factor
Role of fatty acid-binding proteins
proteoglycans and ion transport in differentiation and pathology
Collagen Type I
ACTIVATION
Biomaterials
Glycosaminoglycan
Neovascularization
angiogenesis
chemistry.chemical_compound
Tissue engineering
De rol van vetzuurbindende eiwitten
proteoglycanen en iontransport bij differentiatie en pathologie
GLYCOSAMINOGLYCAN
medicine
Animals
Regeneration
FIBROBLAST-GROWTH-FACTOR
PROTEOGLYCANS
NEOVASCULARIZATION
AFFINITY
RELEASE
Drug Implants
Chemistry
Physical
Heparin
Growth factor
growth factor
IN-VITRO
Heparan sulfate
Immunohistochemistry
Stimulation
Chemical
Cell biology
Rats
RECEPTORS
Microscopy
Electron
Cross-Linking Reagents
chemistry
tissue engineering
Fibroblast Growth Factor 2
heparan sulfate
medicine.symptom
Biomedical engineering
Zdroj: Journal of Biomedical Materials Research, 62, 185-94
Journal of Biomedical Materials Research, 62, 2, pp. 185-94
Journal of Biomedical Materials Research, 62(2), 185-194. Wiley
ISSN: 0021-9304
Popis: The loading of biocompatible matrices with growth factors offers the opportunity to induce specific cell behavior. The attachment of heparan sulfate (HS) to these matrices may promote the binding, modulation, and sustained release of signaling molecules. In this study, basic fibroblast growth factor (bFGF) was bound to crosslinked collagenous matrices with and without covalently attached HS. The tissue response to these matrices was evaluated after subcutaneous implantation in rats. Attachment of HS to collagen matrices increased the bFGF binding capacity threefold and resulted in a more gradual and sustained release of the growth factor in vitro. bFGF primarily was located at the matrix margins. In vivo, the presence of HS without bFGF resulted in a transient vascularization, predominantly at the matrix periphery. Angiogenesis was further enhanced by combining HS with bFGF. In contrast to collagen-HS and collagen/bFGF matrices, collagen-HS/ bFGF matrices remained highly vascularized throughout the matrix during the 10-week implantation period. In addition, these latter matrices revealed an intense and prolonged tissue response and considerably promoted the generation of new tissue. Foreign body reactions were only observed sporadically at this time interval. It is concluded that bFGF loading of collagen-HS matrices has additional value for those tissue-engineering applications that require enhanced angiogenesis and generation of new tissue. (C) 2002 Wiley Periodicals, Inc.
Databáze: OpenAIRE
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