Structure of the N-acetyl-l-rhamnosamine-containing O-polysaccharide of Proteus vulgaris TG 155 from a new Proteus serogroup, O55
| Autor: | Krystyna Zych, Sof'ya N. Senchenkova, Alexander S. Shashkov, Katarzyna Kołodziejska, Yuriy A. Knirel, Zygmunt Sidorczyk, Anna N. Kondakova |
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| Rok vydání: | 2003 |
| Předmět: |
Magnetic Resonance Spectroscopy
Molecular Sequence Data Proteus vulgaris Mannose Polysaccharide Rhamnose Biochemistry Analytical Chemistry chemistry.chemical_compound Animals Monosaccharide chemistry.chemical_classification biology Polysaccharides Bacterial Organic Chemistry Amino Sugars General Medicine Nuclear magnetic resonance spectroscopy biology.organism_classification Proteus Carbohydrate Sequence chemistry Polyclonal antibodies biology.protein Rabbits Heteronuclear single quantum coherence spectroscopy |
| Zdroj: | Carbohydrate Research. 338:1999-2004 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/s0008-6215(03)00327-6 |
| Popis: | The O-polysaccharide of the lipopolysaccharide (LPS) of Proteusvulgaris TG 155 was found to contain 2-acetamido-2,6-dideoxy- l -mannose (N-acetyl- l -rhamnosamine, l -RhaNAc), a monosaccharide that occurs rarely in Nature. The following structure of the O-polysaccharide was established by NMR spectroscopy, including 2D COSY, TOCSY, ROESY and 1H,13C HSQC experiments, along with chemical methods: Download : Download full-size image Rabbit polyclonal O-antiserum against P. vulgaris TG 155 reacted with both core and O-polysaccharide moieties of the homologous LPS but showed no cross-reactivity with other LPS from the complete set of serologically different Proteus strains. Based on the unique O-polysaccharide structure and the serological data, we propose classifying P.vulgaris TG 155 into a new, separate Proteus O-serogroup, O55. |
| Databáze: | OpenAIRE |
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