hucMSCs transplantation promotes locomotor function recovery, reduces apoptosis and inhibits demyelination after SCI in rats
| Autor: | Xiuzhen Yang, Ziling Liao, Yuying Zhang, Weiyue Deng, Zhiyuan Li, Aishi Song, Bin Ni, Wei Wang, Huifang Zhao, Shusheng Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
030209 endocrinology & metabolism Apoptosis Mesenchymal Stem Cell Transplantation Umbilical cord Andrology Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience symbols.namesake 0302 clinical medicine Endocrinology medicine Animals Humans Spinal cord injury Spinal Cord Injuries Neurons biology Endocrine and Autonomic Systems business.industry Myelin Basic Protein General Medicine Recovery of Function medicine.disease Spinal cord Oligodendrocyte Myelin basic protein Rats Transplantation Oligodendroglia medicine.anatomical_structure Neurology Gene Expression Regulation Blood-Brain Barrier Nissl body symbols biology.protein Female business 030217 neurology & neurosurgery Locomotion Demyelinating Diseases |
| Zdroj: | Neuropeptides. 86 |
| ISSN: | 1532-2785 |
| Popis: | Aims Spinal cord injury (SCI) can cause a variety of cells apoptosis, neurodegeneration, and eventually permanent paralysis. This study aimed to examine whether transplanting human umbilical cord mesenchymal stem cells (hucMSCs) can promote locomotor function recovery, reduce apoptosis and inhibit demyelination in SCI models. Main methods Rats were allocated into Sham group (spinal cord exposure only), SCI + PBS group (spinal cord impact plus phosphate-buffered saline (PBS) injections), SCI + hucMSCs group (spinal cord impact plus hucMSCs injections) groups. Behavioral tests, Basso-Beattie-Bresnahan locomotion scores (BBB scores), were carried out at 0, 3, 7, 14, 21, 28 days after SCI surgery. Hematoxylin-eosin staining observed spinal cord morphology. Nissl staining detected the number of nissl bodies. Myelin basic protein (MBP) and oligodendrocyte (CNPase) were examed by immunohistochemical staining. The apoptosis of oligodendrocyte and neurons were detected by immunofluorescence. Results The 28-day behavioral test showed that the BBB score of rats in the SCI + hucMSCs group increased significantly, comparing to the SCI + PBS group. The numbers of nissl bodies and myelin sheath in the damaged area of SCI + hucMSCs group were also significantly increased compared to the SCI + PBS group. HucMSCs transplanting decreased the expression of protein level of Caspase-3 and Bax and increased the Bcl-2, MBP and CNPase, rescued the apoptosis of neurons and the oligodendrocyte. Conclusion These results showed that hucMSCs can improve motor function, tissue repairing and reducing apoptosis in SCI rats. |
| Databáze: | OpenAIRE |
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