Isolation of a selective marker from Cephalosporium acremonium by complementation of an auxotrophic mutant of E. coli

Autor:	E. Friedlin, J. Nüesch
Rok vydání:	1984
Předmět:	Genetics Acremonium Auxotrophy fungi Genetic transfer General Medicine Biology biology.organism_classification Complementation Shuttle vector Cosmid Vector Cosmid bacteria Genomic library
Zdroj:	Current Genetics. 8:271-276
ISSN:	1432-0983 0172-8083
Popis:	A Cephalosoporium acremonium gene library has been constructed using cosmid vector pYcl. After transformation of E. coli leuB strain JA221 with cosmids isolated from this gene library a cosmid (pCeleuB43) was isolated which complements the leucine auxotrophy of JA221. The same cosmid is also able to complement a leu2 strain of S. cerevisiae. The leuB and leu2 genes both code for β-isopropylmalate dehydrogenase. The complementation of a leuB E. coli strain and a leu2 S. cerevisiae strain strongly suggests that the analogous gene from C. acremonium has been isolated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::858cf23eda0aa5be7c9fdfa0353cfd89 https://doi.org/10.1007/bf00419724 Zobrazit plný text záznamu Full text from SpringerLink