α-Defensin 1 (Human Neutrophil Protein 1) as an Antichemotactic Agent for Human Polymorphonuclear Leukocytes
| Autor: | Patricia S. Grutkoski, C. T. Graeber, H. Hank Simms, A. Ayala, Y. P. Lim |
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| Rok vydání: | 2003 |
| Předmět: |
alpha-Defensins
Human neutrophil Neutrophils Blotting Western In Vitro Techniques Receptors Interleukin-8A law.invention chemistry.chemical_compound law Humans Pharmacology (medical) Interleukin 8 α defensin Biologic Response Modifiers Chromatography High Pressure Liquid Pharmacology Tumor Necrosis Factor-alpha Interleukin-8 hemic and immune systems N-Formylmethionine leucyl-phenylalanine Blotting western Molecular biology Recombinant Proteins N-Formylmethionine Leucyl-Phenylalanine Chemotaxis Leukocyte Infectious Diseases chemistry Biochemistry Protein human Recombinant DNA Tumor necrosis factor alpha |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 47:2666-2668 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.47.8.2666-2668.2003 |
| Popis: | Medium conditioned by tumor necrosis factor alpha (TNF-α)-stimulated polymorphonuclear leukocytes (PMN) (CM-TNF) suppresses PMN migration. Therefore, we wished to identify the agent(s) in CM-TNF that mediated antichemotactic activity. CM-TNF was fractionated by high-performance liquid chromatography, and one fraction with antichemotactic activity contained the bactericidal protein human neutrophil protein 1 (HNP-1). We showed that HNP-1 suppresses PMN migration to formyl-methionyl-leucyl-phenylalanine but not to interleukin 8. |
| Databáze: | OpenAIRE |
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